DAB Substrate Kit from Abcam

Product Specs
ItemDAB Substrate Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab64238
Quantity60 ML, 125 ML
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TargetDAB substrate kit

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Urolithin A protects severe acute pancreatitis-associated acute cardiac injury by regulating mitochondrial fatty acid oxidative metabolism in cardiomyocytesMedComm (2020)December 19, 2023Yue Yang, Qian Hu, Hongxin Kang, Juan Li, Xianlin Zhao, Lv Zhu, Wenfu Tang, Meihua Wan37°C for 1 h. Subsequently, the color development process utilized DAB color development solution (ab64238; Abcam), and hematoxylin was applied for counterstaining. The 3DHISTECH panoramic SCAN instrument was

(2) Helicobacter pylori-induced fibroblast-derived Serpin E1 promotes gastric cancer growth and peritoneal dissemination through p38 MAPK/VEGFA-mediated angiogenesisCancer Cell IntDecember 16, 2023Wei Cheng, Yonghui Liao, Yuan Xie, Qinrong Wang, Leilei Li, Yuanjia Chen, Yan Zhao, Jianjiang ZhouHRP-conjugated secondary antibodies at room temperature. The signals were then visualized with a DAB kit (ab64238, Abcam, UK), The DAB-visualized slices were counterstained in hematoxylin, acid alcohol, and deionized

(3) A mutual regulatory loop between miR-155 and SOCS1 influences renal inflammation and diabetic kidney diseaseMolecular Therapy - Nucleic AcidsDecember 12, 2023Ignacio Prieto, María Kavanagh, Luna Jimenez-Castilla, Marisa Pardines, Iolanda Lazaro, Isabel Herrero del Real, Monica Flores-Muñoz, Jesus Egido, Oscar Lopez-Franco, Carmen Gomez-Guerrerofor 45 min. Positive signal was visualized by the addition of 3,3′-diaminobenzidine substrate (DAB; ab64238; Abcam) and samples were counterstained with hematoxylin. Positive staining (>10 fields at 20× magnification;

(4) Immunoexpression of Spexin in Selected Segments of the Bovine (Bos taurus taurus) Gastrointestinal TractAnimals (Basel)December 8, 2023Aleksandra Dajnowska, Cezary Osiak-Wicha, Małgorzata Piech, Siemowit Muszyński, Ewa Tomaszewska, Katarzyna Ropka-Molik, Michał K. Krzysiak, Marcin B. Arciszewskidevelopment of sections was initiated by immersing them in 3,3′Diaminobenzidine (DAB substrate kit, ab64238 Abcam, Cambridge, UK) until a brown coloration was achieved. The reaction was halted by immersing the

(5) Long-Term Survival of Patients with Stage T1N0M1 Renal Cell CarcinomaCancers (Basel)December 6, 2023Viktoria Schütz, Huan Lin, Adam Kaczorowski, Stefanie Zschäbitz, Dirk Jäger, Albrecht Stenzinger, Anette Duensing, Jürgen Debus, Markus Hohenfellner, Stefan Duensingroom temperature for 30 min. Slides were then stained with DAB solution (DAB Substrate Kit, Abcam, ab64238) and transferred into tap water. Counterstaining was performed with hematoxylin for 5 s (Hematoxylin

(6) Breast cancer stem cells generate immune-suppressive T regulatory cells by secreting TGFβ to evade immune-eliminationDiscov OncolDecember 1, 2023Sumon Mukherjee, Sourio Chakraborty, Udit Basak, Subhadip Pati, Apratim Dutta, Saikat Dutta, Dia Roy, Shruti Banerjee, Arpan Ray, Gaurisankar Sa, et al.respectively) for 1 h, treated with the chromogenic substance 3,3′-diaminobenzidine (Abcam, Cat. No. ab64238), and thereafter counter-staining was done in hematoxylin (SRL, India). The slides were washed, dehydrated,

(7) Hammerhead-type FXR agonists induce an eRNA FincoR that ameliorates nonalcoholic steatohepatitis in micebioRxivNovember 24, 2023Jinjing Chen, Ruoyu Wang, Feng Xiong, Hao Sun, Byron Kemper, Wenbo Li, Jongsook Kim Kemperwere incubated with F4/80 antibody, and antibody was detected using a peroxidase-based method (Abcam, ab64238). Liver collagen was detected by Sirius Red staining (Abcam, ab246832) and apoptosis was detected by

(8) Hepatic macrophages play critical roles in the establishment and growth of hydatid cysts in the liver during Echinococcus granulosus sensu stricto infectionPLOS Neglected Tropical DiseasesNovember 6, 2023Hui Wang, Qian Yu, Mingkun Wang, Jiao Hou, Maolin Wang, Xuejiao Kang, Xinling Hou, Dewei Li, Zibigu Rousu, Tiemin Jiang, et al.room temperature. Visualization of staining was performed with a diaminobenzidine (DAB) substrate kit (ab64238, Abcam, Cambridge, UK) according to the manufacturer’s instructions. Then, the stained sections were

(9) Molecular mechanisms of AMPK/YAP/NLRP3 signaling pathway affecting the occurrence and development of ankylosing spondylitisJournal of Orthopaedic Surgery and ResearchNovember 4, 2023Ruiyang Fu, Xiaoqing Guo, Zhongqiang Pan, Yaling Wang, Jing Xu, Lei Zhang, Jinxia Lisecondary antibody (#8114, 1/5000; CST) for the deparaffinized tissue sections. Use DAB staining reagent (ab64238, Abcam, Shanghai, China) to visualize the binding site between the secondary and primary antibodies.

(10) Expression of genes and enzymes involved in ovarian steroidogenesis in relation to human follicular developmentFront Endocrinol (Lausanne)October 26, 2023Mengxue Zheng, Claus Yding Andersen, Frida Roikjer Rasmussen, Jesús Cadenas, Søren Tvorup Christensen, Linn Salto Mamsenperoxidase reaction using 3,3’-diaminobenzidine tetrahydrochloride (DAB substrate kit Abcam, Cat. No. ab64238). 2.8 Fluorescent immunohistochemistry The procedure for immunofluorescence was carried out essentially

(11) Combining bioengineered human skin with bioprinted cartilage for ear reconstructionScience AdvancesOctober 6, 2023Dominika Zielinska, Philipp Fisch, Ueli Moehrlen, Sergio Finkielsztein, Thomas Linder, Marcy Zenobi-Wong, Thomas Biedermann, Agnes S. Klardiluted in 1% BSA in PBS for 1 hour. Chromogen development was performed with the DAB substrate kit (ab64238, Abcam) according to the manufacturer specifications for 5 min. Last, sections were stained with Weigert’s

(12) Effect of glycosaminoglycans on the structure and composition of articular cartilage and bone of broilersPoultry ScienceOctober 1, 2023Julyana M.S. Martins, Lindolfo D. dos Santos Neto, Sarah Sgavioli, Itallo C.S. Araújo, Angela A.S. Reis, Rodrigo S. Santos, Eugênio G. de Araújo, Nadja S.M. Leandro, Marcos B. CaféThe reaction was revealed with the addition of the chromogen diaminobenzidine-peroxidase (DAB, Abcam, ab64238, GR313581-2, Cambridge, England) for 4 min. After that, the slides were washed with PBS for 5 min, dehydrated

(13) Regulation of Osteoimmune Microenvironment and Osteogenesis by 3D‐Printed PLAG/black Phosphorus Scaffolds for Bone RegenerationAdvanced ScienceOctober 1, 2023Jing Long, Zhenyu Yao, Wei Zhang, Ben Liu, Kaiming Chen, Long Li, Bin Teng, Xiang‐Fu Du, Cairong Li, Xue‐Feng Yu, et al.incubated at room temperature for 1 h with the slides the following day. Then, the DAB solution (Abcam, ab64238) was dropped onto the sections to visualize the color of the antibody staining, and hematoxylin was

(14) Microencapsulated stem cells reduce cartilage damage in a material dependent manner following minimally invasive intra-articular injection in an OA rat modelMaterials Today BioOctober 1, 2023Castro Johnbosco, Lisanne Karbaat, Nicoline M. Korthagen, Kelly Warmink, Michelle Koerselman, Katja Coeleveld, Malin Becker, Bas van Loo, Bram Zoetebier, Sanne Both, et al.P0447) was incubated for 1 h at room temperature, and the staining was visualized using DAB (Abcam ab64238). For the determination of the OARSI scoring, slides of the load-bearing areas of the joint were rehydrated,

(15) The rejuvenating influence of young plasma on aged intestineJournal of Cellular and Molecular MedicineSeptember 1, 2023Taha Ceylani, Hikmet Taner Teker, Seda Keskin, Gizem Samgane, Eda Acikgoz, Rafig Gurbanovstreptavidin peroxidase (TS‐125‐HR) were applied to the sections. Then, a 3,3′‐Diaminobenzidine substrate kit (ab64238, Abcam) was performed for 3–5 min. Finally, the sections were counterstained with Mayer's haematoxylin

(16) Early Degenerative Changes in a Spontaneous Osteoarthritis Model Assessed by NanoindentationBioengineering (Basel)August 23, 2023Sarah Davis, Jurgita Zekonyte, Aikaterina Karali, Marta Roldo, Gordon Blunn(1:2000; ab205719; Abcam, Cambridge, UK) for 1 h at RT followed by DAB (3,3′Diaminobenzidine) chromogen (ab64238; Abcam, Cambridge, UK). Sections were counterstained with Mayer’s Haemaoxylin (MH51; HT110116; Sigma-Aldrich,

(17) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal ModelPharmaceuticals (Basel)August 22, 2023Shan-Chih Lee, Chao-Yu Shen, Wei-Hsun Wang, Yen-Po Lee, Keng-Wei Liang, Ying-Hsiang Chou, Yeu-Sheng Tyan, Jeng-Jong Hwangin paraffin, sectioned at 5 μm, and stained with 3′,3′-diaminobenzidine (DAB) substrate kit (Abcam, ab64238), and counterstained with Mayer’s hematoxylin (ScyTek Laboratories, UT, USA). All sections were scanned

(18) Epigenetic deregulation of MLF1 drives intrahepatic cholangiocarcinoma progression through EGFR/AKT and Wnt/β-catenin signalingHepatology CommunicationsAugust 1, 2023Zengwei Tang, Yuan Yang, Wen Chen, Tingbo Liangthen incubated with biotinylated secondary antibody before visualization using DAB substrate (Abcam, ab64238). Immunofluorescence Immunofluorescence was performed as described.3 After blocking with 1% bovine

(19) A Cell Cycle-Dependent Ferroptosis Sensitivity Switch Governed by EMP2bioRxiv : the preprint server for biologyJuly 19, 2023Rodencal J, Kim N, Li VL, He A, Lange M, He J, Tarangelo A, Schafer ZT, Olzmann JA, Sage J, et al.dilution for 45 min at RT. Slides were washed three times with TBS for 3 min each. DAB chromogen (Cat# ab64238, AbCam) was added to the slides for 5 min, and slides were washed in deionized water for 1 min. Slides

(20) Scopolamine causes delirium-like brain network dysfunction and reversible cognitive impairment without neuronal lossZoological ResearchJuly 18, 2023Qing Wang, 四川大学华西医院国家老年医学临床研究中心老年医学科, 生物治疗国家重点实验室, 四川成都 610041, 中国, Department of Geriatrics and State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China, Xiang Zhang, Yu-Jie Guo, Ya-Yan Pang, Jun-Jie Li, Yan-Li Zhao, Jun-Fen Wei, Bai-Ting Zhu, et al.(Thermo Fisher Scientific, USA, 31460) and chromogenic substrate 3,3′-diaminobenzidine (Abcam, USA, ab64238). The sections were then dehydrated through gradient alcohol (50%, 75%, 95%, and 100%) and cover-slipped

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DAB Substrate Kit from Abcam

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DAB Substrate Kit from Abcam

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